Antigen

Antigen Class

TFs and others

Antigen

Creb1

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Fed 24 hrs pCREB ChIP-seq

strain

C57BL/6

tissue

liver

chip antibody

Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

crosslinked material was lyzed and sonicated. Chromatin precipitated with antibody and DNA was purified by ethanol precipitation. All the sequencing data were sequenced on NextSeq 500 using NextSeq High V2 chemistry single end.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

25886589

Reads aligned (%)

96.8

Duplicates removed (%)

18.6

Number of peaks

1551 (qval < 1E-05)

mm9

Number of total reads

25886589

Reads aligned (%)

96.6

Duplicates removed (%)

18.6

Number of peaks

1545 (qval < 1E-05)